SE-HPLC separation of myosin complex with tannins from bearberries (Arctostaphylos uva-ursi L. Sprengel) leaves – a short communication

To chci

Phenolic compounds were extracted from bearberry (Arctostaphylos uva-ursi L. Sprengel) leaves into 95% (v/v) ethanol. The tannin constituents were separated from the crude extract using Sephadex LH-20 column chromatography with 95% (v/v) ethanol and 50% (v/v) acetone as the mobile phases. Myosin was isolated and purified from excised pork knuckle muscles using the standard salt-solution extraction procedure followed by gel filtration chromatography. Myosin was precipitated from the solution with bearberry-leaf tannins at pH 5.0. The recovered complex was washed, lyophilised, and subjected to size-exclusion high performance liquid chromatography (SE-HPLC). Based on the basic conditions of the HPLC analysis, a portion of the tannin constituents was liberated from the complex. The UV spectra of these compounds were characterized by a maximum at ~ 300 nm. A portion of the tannins was present in the complex with myosin, and this was confirmed by UV spectra. … celý popis

Podrobná bibliografie

Hlavní autor

Ryszard Amarowicz

Další autoři

Ronald B. Pegg
Agnieszka Kosińska

Typ dokumentu

Články

Fyzický popis

3 il.

Publikováno v

Czech Journal of Food Sciences. -- ISSN 1212-1800. -- Roč. 27, č. 5 (2009), s. 386-391

Témata

LEAVES

MYOSIN

TANNINS

SEPARATING

PHENOLIC COMPOUNDS

GLUCOSIDES

CHROMATOGRAPHY

medvědice lékařská

Arctostaphylos uva-ursi

listy

myosin

taniny

separace

fenolové sloučeniny

glukosidy

bílkoviny

chromatografie

Popis jednotky

Grafy

Bibliografie

Bibliografie na s. 390-391 (27 zázn.)