TLC separation of methylated (-)-epigallocatechin-3-gallate
Methylated EGCG was separated from the crude extract using Sephadex LH-20 column chromatography with methanol as the mobile phase, and a semi-preparative HPLC method with water-dimethylformamide-methanol-acetic acid (157:40:2:1, v/v/v/v) as the mobile phase. The chemical structure of the separated catechin was confirmed by ESI-MS in the negative-ion mode. Three … celý popis
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- Články
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- 3 grafy, 2 tab
- Publikováno v
- Czech Journal of Food Sciences. -- ISSN 1212-1800. -- Roč. 23, č. 1, (2005), s. 36-39
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- Popis jednotky
- Lit. s. 38 (24 zázn.)
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Česká zemědělská a potravinářská bibliografie
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MARC
| LEADER | 00000naa a2200000 a 4500 | ||
|---|---|---|---|
| 001 | czpb000086177 | ||
| FMT | A | N | |
| LDR | | naa a22 a 4500 | ||
| 003 | CZ PrUZP | ||
| 008 | 050529s2005 xr f eng | ||
| 040 | |a ABA009 |b cze | ||
| 080 | |a 663.95 |2 undef | ||
| 100 | 1 | |a Amarowicz, Ryszard, |u Polish Academy of Sciences, Olsztyn, PL | |
| 242 | 1 | 0 | |a Separace metylované (-)-epigalokatechin-3-galové kyseliny metodou TLC |
| 245 | 1 | 0 | |a TLC separation of methylated (-)-epigallocatechin-3-gallate / |c Ryszard Amarowicz |
| 300 | |b 3 grafy, 2 tab | ||
| 500 | |a Lit. s. 38 (24 zázn.) | ||
| 520 | |a Methylated EGCG was separated from the crude extract using Sephadex LH-20 column chromatography with methanol as the mobile phase, and a semi-preparative HPLC method with water-dimethylformamide-methanol-acetic acid (157:40:2:1, v/v/v/v) as the mobile phase. The chemical structure of the separated catechin was confirmed by ESI-MS in the negative-ion mode. Three different mobile phases were used for silica gel and reversed phase TLC of EGCG and methylated EGCG. Rf values of both catechins were calculated and are reported. In the normal phase, the best condition of separation was with chloroform-methanol-water (65:35:10; v/v/v; lower phase) being used as the mobile phase. On octadodecylsilanised silica gel plates, the phase water-acetonitrile-methanol-acetic acid (79.5:18:2:0.5; v/v/v/v) offered the best separation of catechins. |9 eng | ||
| 650 | 0 | 9 | |a ANALYTICAL METHODS |2 agrovoc |
| 650 | 0 | 9 | |a HPLC |2 agrovoc |
| 650 | 0 | 9 | |a ION EXCHANGE CHROMATOGRAPHY |2 agrovoc |
| 650 | 0 | 9 | |a MASS SPECTROMETRY |2 agrovoc |
| 650 | 0 | 9 | |a TANNINS |2 agrovoc |
| 650 | 0 | 9 | |a TEA |2 agrovoc |
| 650 | 0 | 7 | |a HPLC |2 agrovoc |
| 650 | 0 | 7 | |a analytické metody |2 agrovoc |
| 650 | 0 | 7 | |a chromatografie na měničích iontů |2 agrovoc |
| 650 | 0 | 7 | |a hmotnostní spektrometrie |2 agrovoc |
| 650 | 0 | 7 | |a taniny |2 agrovoc |
| 650 | 0 | 7 | |a čaj |2 agrovoc |
| 653 | 0 | |a katechiny | |
| 653 | 0 | |a kyselina galová | |
| 653 | 0 | |a zelený čaj | |
| 700 | 1 | |a Maryniak, Anna, |u Polish Academy of Sciences, Olsztyn, PL | |
| 700 | 1 | |a Shahidi, Fereidoon, |u Memorial University of Newfoundland, St John´s, CA | |
| 773 | 0 | |t Czech Journal of Food Sciences |x 1212-1800 |g Roč. 23, č. 1, (2005), s. 36-39 |q 23:1 |9 2005 | |
| LKR | |a ITM |b 97096 |l UZP50 |y 2005 | ||
| CAS | |a a | ||
| SIF | |z Old |d 20050304 |9 2 | ||
| 910 | |a ABA009 |t rs | ||