Cytological investigation of anther development in DGMS line Shaan-GMS in Brassica napus L.

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The cytological mechanism of male sterility of Shaan-GMS, a natural mutant dominant genic male sterile (DGMS) line in Brassica napus, is not well studied. Cytological observation was made on different-size buds of DGMS line 0A30A derived from Shaan-GMS line. The pollen mother cells (PMCs) of DGMS line 0A30A were degenerating at the beginning of meiosis and could not pass the anaphase I stage, with no dyads or tetrads formed, suggesting that the DNA damage checkpoint and spindle assembly checkpoint were activated in sterile anthers. During the meiosis process of sterile anthers in the sterile plants, several kinds of abnormal meiotic cells could be observed: nuclei condensed PMCs, cells with micronuclei, collapsed cells, plasmolysis cells, cells connected with nucleoplasmic bridge, and microspore analogue developed from PMCs without meiosis but enclosed by the exine wall. The results suggested Shaan-GMS to be a new type of DGMS line in B. napus. … full description

Bibliographic Details

Main Author

Zhao Di Xiao

Other Authors

Xiao Yang Xin
Hai Yan Chen
Sheng Wu Hu

Document Type

Articles

Physical Description

5 ilustrací

Published in

Czech Journal of Genetics and Plant Breeding. -- ISSN 1212-1975. -- Roč. 49, č. 1 (2013), s. 16-23

Subjects

OIL CROPS

BRASSICA NAPUS

REPRODUCTIVE PERFORMANCE

MUTATION

Breeding lines

HYBRIDIZATION

HETEROSIS BREEDING

FLOWERS

POLLINATION

GENETIC DISORDERS

CYTOLOGY

MALE INFERTILITY

DNA (nukleová kyselina)

MEIOSIS

CHROMOSOME PAIRING

CELL DIVISION

MICROSCOPY

ULTRASTRUCTURE

olejniny

řepka setá

výkonnost plodin

reprodukční schopnost

mutace

šlechtitelské linie

hybridizace

heterózní šlechtění

květy

opylování

prašníky

genetické poruchy

cytologie

samčí neplodnost

meióza

párování chromozómů

dělení buněk

mikroskopie

ultrastruktura

Item Description

3 fotografie, 2 tabulky

Bibliography

Literatura na straně 23,