The effect of copper on plant regeneration in barley microspore culture

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Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture. … full description

Bibliographic Details

Main Author

Katarzyna Makovska

Other Authors

Sylwia Oleszczuk
Janusz Zimny

Document Type

Articles

Physical Description

5 ilustrací

Published in

Czech Journal of Genetics and Plant Breeding. -- ISSN 1212-1975. -- Roč. 53, č. 1 (2017), s. 17-22

Subjects

Breeding lines

GENOTYPES

POT EXPERIMENTATION

CULTIVATION

BIOTECHNOLOGY

HAPLOMETHODS

DIPLOIDY

ANDROGENESIS

IN VITRO CULTURE

COPPER SULPHATE

REGENERATIVE ABILITY

VIABILITY

mikrospory

ječmen setý

ječmen

jarní plodiny

šlechtitelské linie

genotypy

albinismus

nádobové pokusy

kultivace

prašníky

biotechnologie

haploidizace

diploidie

androgeneze

kultura in vitro

síran měďnatý

regenerační schopnost

životaschopnost

Item Description

4 grafy, 1 tabulka

Bibliography

Literatura na straně 21-22,